Laboratory Protocol

Research/laboratory platforms and technical expertise

Molecular Biology: DNA/RNA/microRNA manipulation and preparation, qPCR, cloning, CRISPR/CAS9 knockout, CRISPR/Cas Activation Systems, Lentiviral packaging and production for shRNAs and CRISPR constructs, Luciferase, cell viability, apoptosis, autophagy, cell cycle regulation assays...

Signal Transduction: Cell culture (cancer and primary cells), Live imaging, Microscopy, Western Blotting, Co-Immunoprecipitation, ELISA, Immunohistochemistry...

Genome-wide CRISPR/Cas9 Screens: whole-genome library preparation, lentiviral packaging, viral in vitro and in vivo delivery

Bioinformatics: Illumina 4000 Deep sequencing, RNASeq analysis, Pathway analysis, online database (ATCG,….)...

Pre-Clinical Models: orthotopic mammary fat pad transplantation, pancreatic transplantation, subcutaneous injection, intraperitoneal injection (IP), intravenous injection (IV), tail vein injection, in vivoJet intratumoral drug delivery, primary tumor preparation and analysis (breast, liver, pancreas, ovary, prostate, skin), metastatic tumor preparation and analysis (lung, liver, kidney, bone), IHC, live fluorescent and luminescent bioimaging

Drug Development: small molecule inhibitor design and synthesis, combi-molecules, Computational inhibitor prediction and design...